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- ItemQuantification of internalized silica nanoparticles via STED microscopy(London : Hindawi, 2015) Peuschel, Henrike; Ruckelshausen, Thomas; Cavelius, Christian; Kraegeloh, AnnetteThe development of safe engineered nanoparticles (NPs) requires a detailed understanding of their interaction mechanisms on a cellular level. Therefore, quantification of NP internalization is crucial to predict the potential impact of intracellular NP doses, providing essential information for risk assessment as well as for drug delivery applications. In this study, the internalization of 25 nm and 85 nm silica nanoparticles (SNPs) in alveolar type II cells (A549) was quantified by application of super-resolution STED (stimulated emission depletion) microscopy. Cells were exposed to equal particle number concentrations (9.2 x 10^10 particles mL^-1) of each particle size and the sedimentation of particles during exposure was taken into account. Microscopy images revealed that particles of both sizes entered the cells after 5 h incubation in serum supplemented and serum-free medium. According to the in vitro sedimentation, diffusion, and dosimetry (ISDD) model 20–27 of the particles sedimented. In comparison, 102-103 NPs per cell were detected intracellularly serum-containing medium. Furthermore, in the presence of serum, no cytotoxicity was induced by the SNPs. In serum-free medium, large agglomerates of both particle sizes covered the cells whereas only high concentrations (≥ 3.8 × 10^12 particles mL^-1) of the smaller particles induced cytotoxicity.
- ItemGeometric models for isotropic random porous media: A review(London : Hindawi, 2014) Hermann, Helmut; Elsner, AntjeModels for random porous media are considered. The models are isotropic both from the local and the macroscopic point of view; that is, the pores have spherical shape or their surface shows piecewise spherical curvature, and there is no macroscopic gradient of any geometrical feature. Both closed-pore and open-pore systems are discussed. The Poisson grain model, the model of hard spheres packing, and the penetrable sphere model are used; variable size distribution of the pores is included. A parameter is introduced which controls the degree of open-porosity. Besides systems built up by a single solid phase, models for porous media with the internal surface coated by a second phase are treated. Volume fraction, surface area, and correlation functions are given explicitly where applicable; otherwise numerical methods for determination are described. Effective medium theory is applied to calculate physical properties for the models such as isotropic elastic moduli, thermal and electrical conductivity, and static dielectric constant. The methods presented are exemplified by applications: small-angle scattering of systems showing fractal-like behavior in limited ranges of linear dimension, optimization of nanoporous insulating materials, and improvement of properties of open-pore systems by atomic layer deposition of a second phase on the internal surface.
- ItemSynthesis and characterization of aluminum doped zinc oxide nanostructures via hydrothermal route(London : Hindawi, 2014) Alkahlout, Amal; Al Dahoudi, Naji; Grobelsek, Ingrid; Jilavi, Mohammad; Oliveira de, Peter W.Stable crystalline aluminum doped zinc oxide (AZO) nanopowders were synthesized using hydrothermal treatment processing. Three different aluminum precursors have been used. The Al-precursors were found to affect the morphology of the obtained nanopowders. AZO nanoparticles based on zinc acetate and aluminum nitrate have been prepared with different Al/Zn molar ratios. XRD investigations revealed that all the obtained powders have single phase zincite structure with purity of about 99%. The effect of aluminum doping ratio in AZO nanoparticles (based on Al-nitrate precursor) on structure, phase composition, and particle size has been investigated. The incorporation of Al in ZnO was confirmed by UV-Vis spectroscopy revealing a blue shift due to Burstein-Moss effect.
- ItemPlasma rotation with circularly polarized laser pulse(London : Hindawi, 2015) Lécz, Z.; Andreev, A.; Seryi, A.The efficient transfer of angular orbital momentum from circularly polarized laser pulses into ions of solid density targets is investigated with different geometries using particle-in-cell simulations. The detailed electron and ion dynamics presented focus upon the energy and momentum conversion efficiency. It is found that the momentum transfer is more efficient for spiral targets and the maximum value is obtained when the spiral step is equal to twice the laser wavelength. This study reveals that the angular momentum distribution of ions strongly depends up on the initial target shape and density.
- ItemEffects of free surface and heterogeneous residual internal stress on stress-driven grain growth in nanocrystalline metals(London : Hindawi, 2013) Schneider, Andreas S.; Wang, F.; Zhao, J.; Huang, P.; Lu, T.J.; Xu, K.W.By reevaluating the experimental study of Zhang et al. (2005), here we demonstrate that the extent of grain growth, previously proposed to be solely driven by external stress, may have been significantly overestimated. A new physical mechanism, termed as free surface assisted stress-driven grain growth (or self-mechanical annealing), is proposed and discussed in detail. Representing the cooperative effect of free surface and heterogeneous residual internal stress, the proposed mechanism is considered more favorable than the traditional pure stress-driven mechanism for interpreting the abnormal grain growth widely observed in deforming nanocrystalline metals at room temperature.
- ItemDetailed Fluid Inclusion and Stable Isotope Analysis on Deep Carbonates from the North Alpine Foreland Basin to Constrain Paleofluid Evolution(London : Hindawi, 2019) Mraz, Elena; Wolfgramm, Markus; Moeck, Inga; Thuro, KuroschThe recent interest on environmentally friendly energy resources has increased the economic interest on the Upper Jurassic carbonate rocks in the North Alpine Foreland Basin, which serves as a hydrogeothermal reservoir. An economic reservoir use by geothermal fluid extraction and injection requires a decent understanding of porosity–permeability evolution of the deep laying Upper Jurassic strata at depths greater than 2000 m. The analysis of paleofluids caught in cements of the rock mass helps to determine the postdepositional reservoir evolution and fluid migration. Therefore, the high- and low-permeability areas of the Upper Jurassic in the North Alpine Foreland Basin referred to as Molasse Basin were analyzed by means of encountered postdepositional cements to determine the reservoir evolution. The cements were sampled at different hydrocarbon and geothermal wells, as well as at outcrops in the Franconian and Swabian Alb. To determine the composition and temperature of the paleofluids, fluid inclusions and cements of the Upper Jurassic carbonate rocks were analyzed by microthermometry and stable isotope measurements. Since drill cuttings are a rather available sample material compared to drill cores, a new microthermometry measurement method was achieved for the around 1 mm drill cuttings. Salinity and formation temperature of paleofluids in fluid inclusions and isotope data are consistent with previous studies and reveal a 5-stage evolution: the main cementation phases are composed of (I) the early diagenesis in limestones (200-400 m, 40-50°C), (II) early diagenetic dolomitization, and (III) burial dolomitization (1-2 km, II: 40-90°C; III: 70-100°C; 40 g/L NaCl equiv.), and (IV) late burial calcification (IIIa: 110-140°C, IIIb: 140-200°C) linked to tectonic features in the Molasse Basin. In the outcrop samples, a subsequent (V) cementation phase was determined controlled by karstification. In the southwest, an increase in salinity of the fluid inclusions in vein calcites, above the salinity of the Jurassic seawater, highlights the influence of basin fluids (diagenetic, evaporitic). In the other eastern wells, vein calcites have precipitated from a low saline fluid of around 10-20 g/L NaCl equiv. The low salinity and the isotope values support the theory of a continuous influence of descending meteoric fluids. Consequently, the Upper Jurassic seawater has been diluted by a meteoric fluid to a low saline fluid (<1 g/L), especially in areas with high permeability. Here, we show how a better understanding of cementation trajectory at depth can help to generate a better understanding of geothermal usability in deep carbonate reservoirs.
- ItemHow Price-Based Frequency Regulation Impacts Stability in Power Grids: A Complex Network Perspective(London : Hindawi, 2020) Ji, Peng; Zhu, Lipeng; Lu, Chao; Lin, Wei; Kurths, JürgenWith the deregulation of modern power grids, electricity markets are playing a more and more important role in power grid operation and control. However, it is still questionable how the real-time electricity price-based operation affects power grid stability. From a complex network perspective, here we investigate the dynamical interactions between price-based frequency regulations and physical networks, which results in an interesting finding that a local minimum of network stability occurs when the response strength of generators/consumers to the varying price increases. A case study of the real world-based China Southern Power Grid demonstrates the finding and exhibits a feasible approach to network stability enhancement in smart grids. This also provides guidance for potential upgrade and expansion of the current power grids in a cleaner and safer way. © 2020 Peng Ji et al.
- ItemSystems Medicine of Cancer: Bringing Together Clinical Data and Nonlinear Dynamics of Genetic Networks(London : Hindawi, 2016) Blyuss, Konstantin B.; Manchanda, Ranjit; Kurths, Jürgen; Alsaedi, Ahmed; Zaikin, AlexeyEditorial
- ItemFixed-Time Connectivity Preserving Tracking Consensus of Multiagent Systems with Disturbances(London : Hindawi, 2020) Sun, Fenglan; Liu, Peiyong; Kurths, Jürgen; Zhu, WeiThis text studies the fixed-time tracking consensus for nonlinear multiagent systems with disturbances. To make the fixed-time tracking consensus, the distributed control protocol based on the integral sliding mode control is proposed; meanwhile, the adjacent followers can be maintained in a limited sensing range. By using the nonsmooth analysis method, sufficient conditions for the fixed-time consensus together with the upper and lower bounds of convergence time are obtained. An example is given to illustrate the potential correctness of the main results. © 2020 Fenglan Sun et al.
- ItemIdentification and validation of potential new biomarkers for prostate cancer diagnosis and prognosis using 2D-DIGE and MS(London : Hindawi, 2015) Geisler, Cordelia; Gaisa, Nadine T.; Pfister, David; Fuessel, Susanne; Kristiansen, Glen; Braunschweig, Till; Gostek, Sonja; Beine, Birte; Diehl, Hanna C.; Jackson, Angela M.; Borchers, Christoph H.; Heidenreich, Axel; Meyer, Helmut E.; Knüchel, Ruth; Henkel, CorinnaThis study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (P < 0.044) in prostate cancer, while vinculin showed significant upregulation (P < 0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (P = 0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (P = 0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (P = 0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique.