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- ItemTowards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes(New York, NY [u.a.] : Hindawi Publ. Corp., 2017) Menzel, Sarah; Finocchiaro, Nicole; Donay, Christine; Thiebes, Anja Lena; Hesselmann, Felix; Arens, Jutta; Djeljadini, Suzana; Wessling, Matthias; Schmitz-Rode, Thomas; Jockenhoevel, Stefan; Cornelissen, Christian GabrielIn patients with respiratory failure, extracorporeal lung support can ensure the vital gas exchange via gas permeable membranes but its application is restricted by limited long-term stability and hemocompatibility of the gas permeable membranes, which are in contact with the blood. Endothelial cells lining these membranes promise physiological hemocompatibility and should enable prolonged application. However, the endothelial cells increase the diffusion barrier of the blood-gas interface and thus affect gas transfer. In this study, we evaluated how the endothelial cells affect the gas exchange to optimize performance while maintaining an integral cell layer. Human umbilical vein endothelial cells were seeded on gas permeable cell culture membranes and cultivated in a custom-made bioreactor. Oxygen transfer rates of blank and endothelialized membranes in endothelial culture medium were determined. Cell morphology was assessed by microscopy and immunohistochemistry. Both setups provided oxygenation of the test fluid featuring small standard deviations of the measurements. Throughout the measuring range, the endothelial cells seem to promote gas transfer to a certain extent exceeding the blank membranes gas transfer performance by up to 120%. Although the underlying principles hereof still need to be clarified, the results represent a significant step towards the development of a biohybrid lung.
- ItemA numerical sensitivity study of how permeability, porosity, geological structure, and hydraulic gradient control the lifetime of a geothermal reservoir(Göttingen : Copernicus Publ., 2019) Bauer, Johanna F.; Krumbholz, Michael; Luijendijk, Elco; Tanner, David C.Geothermal energy is an important and sustainable resource that has more potential than is currently utilized. Whether or not a deep geothermal resource can be exploited, mostly depends on, besides temperature, the utilizable reservoir volume over time, which in turn largely depends on petrophysical parameters. We show, using over 1000 (n=1027) 4-D finite-element models of a simple geothermal doublet, that the lifetime of a reservoir is a complex function of its geological parameters, their heterogeneity, and the background hydraulic gradient (BHG). In our models, we test the effects of porosity, permeability, and BHG in an isotropic medium. Furthermore, we simulate the effect of permeability contrast and anisotropy induced by layering, fractures, and a fault. We quantify the lifetime of the reservoir by measuring the time to thermal breakthrough, i.e. how many years pass before the temperature of the produced fluid falls below the 100 ∘C threshold. The results of our sensitivity study attest to the positive effect of high porosity; however, high permeability and BHG can combine to outperform the former. Particular configurations of all the parameters can cause either early thermal breakthrough or extreme longevity of the reservoir. For example, the presence of high-permeability fractures, e.g. in a fault damage zone, can provide initially high yields, but it channels fluid flow and therefore dramatically restricts the exploitable reservoir volume. We demonstrate that the magnitude and orientation of the BHG, provided permeability is sufficiently high, are the prime parameters that affect the lifetime of a reservoir. Our numerical experiments show also that BHGs (low and high) can be outperformed by comparatively small variations in permeability contrast (103) and fracture-induced permeability anisotropy (101) that thus strongly affect the performance of geothermal reservoirs.