2019, Lerra, L., Farfalla, A., Sanz, B., Cirillo, G., Vittorio, O., Voli, F., Grand, M.L., Curcio, M., Nicoletta, F.P., Dubrovska, A., Hampel, S., Iemma, F., Goya, G.F.

With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin–human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the MNPs@GO system and albumin bioconjugate, consisted of MNPs@GO nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44–0.88 µM), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site.

2023, Madeo, Lorenzo Francesco, Curcio, Manuela, Iemma, Francesca, Nicoletta, Fiore Pasquale, Hampel, Silke, Cirillo, Giuseppe

To investigate the influence of crosslinking methods on the releasing performance of hybrid hydrogels, we synthesized two systems consisting of Graphene oxide (GO) as a functional element and alginate as polymer counterpart by means of ionic gelation (physical method, 𝐻𝑃𝐴−𝐺𝑂) and radical polymerization (chemical method, 𝐻𝐶𝐴−𝐺𝑂). Formulations were optimized to maximize the GO content (2.0 and 1.15% for 𝐻𝑃𝐴−𝐺𝑂 and 𝐻𝐶𝐴−𝐺𝑂, respectively) and Curcumin (CUR) was loaded as a model drug at 2.5, 5.0, and 7.5% (by weight). The physico-chemical characterization confirmed the homogeneous incorporation of GO within the polymer network and the enhanced thermal stability of hybrid vs. blank hydrogels. The determination of swelling profiles showed a higher swelling degree for 𝐻𝐶𝐴−𝐺𝑂 and a marked pH responsivity due to the COOH functionalities. Moreover, the application of external voltages modified the water affinity of 𝐻𝐶𝐴−𝐺𝑂, while they accelerated the degradation of 𝐻𝑃𝐴−𝐺𝑂 due to the disruption of the crosslinking points and the partial dissolution of alginate. The evaluation of release profiles, extensively analysed by the application of semi-empirical mathematical models, showed a sustained release from hybrid hydrogels, and the possibility to modulate the releasing amount and rate by electro-stimulation of 𝐻𝐶𝐴−𝐺𝑂.