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Title: Gelation kinetics of thiol-methylsulfone (MS) hydrogel formulations for 3D cell culture
Authors: de Miguel-Jiménez, AdriánEbeling, BastianPaez, Julieta I.Fink-Straube, ClaudiaPearson, Samueldel Campo, Aranzazu
Publishers version: https://doi.org/10.26434/chemrxiv-2022-svh1x
URI: https://oa.tib.eu/renate/handle/123456789/11492
http://dx.doi.org/10.34657/10526
Issue Date: 2022
Published in: ChemRxiv
Journal: ChemRxiv
Publisher: Washington, D.C. : American Chemical Society
Abstract: Crosslinking chemistries that allow hydrogel formation within minutes are essential to achieve homogeneous networks and cell distributions in 3D cell culture. Thiol-methylsulfone (MS) crosslinking chemistry offers minutes-scale gelation under near-physiological conditions showing many desirable attributes for 3D cell encapsulation. Here we investigate the gelation kinetics and mechanical properties of PEG-based hydrogels formed by thiol-tetrazole methylsulfone (TzMS) crosslinking as a function of buffer, crosslinker structure, and degree of TzMS functionalization. Appropriate buffer selection ensured constant pH throughout crosslinking. The formulation containing cell adhesive ligand RGD and enzymatically-degradable peptide VPM gelled in ca. 4 min at pH 7.5, and stiffness could be increased from hundreds of Pascals to > 1 kPa by using excess VPM. The gelation times and stiffnesses for these hydrogels are highly suitable for 3D cell encapsulations, and pave the way for reliable 3D cell culture workflows in pipetting robots.
Keywords: hydrogel; cell encapsulation; cell culture; crosslinking; gelation
Type: workingPaper; Text
Publishing status: draft
DDC: 540
License: CC BY-NC-ND 4.0 Unported
Link to license: https://creativecommons.org/licenses/by-nc-nd/4.0/
Appears in Collections:Chemie

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de Miguel-Jiménez, Adrián, Bastian Ebeling, Julieta I. Paez, Claudia Fink-Straube, Samuel Pearson and Aranzazu del Campo, 2022. Gelation kinetics of thiol-methylsulfone (MS) hydrogel formulations for 3D cell culture. 2022. Washington, D.C. : American Chemical Society
de Miguel-Jiménez, A., Ebeling, B., Paez, J. I., Fink-Straube, C., Pearson, S. and del Campo, A. (2022) “Gelation kinetics of thiol-methylsulfone (MS) hydrogel formulations for 3D cell culture.” Washington, D.C. : American Chemical Society. doi: https://doi.org/10.26434/chemrxiv-2022-svh1x.
de Miguel-Jiménez A, Ebeling B, Paez J I, Fink-Straube C, Pearson S, del Campo A. Gelation kinetics of thiol-methylsulfone (MS) hydrogel formulations for 3D cell culture. Washington, D.C. : American Chemical Society; 2022.
de Miguel-Jiménez, A., Ebeling, B., Paez, J. I., Fink-Straube, C., Pearson, S., & del Campo, A. (2022). Gelation kinetics of thiol-methylsulfone (MS) hydrogel formulations for 3D cell culture (Version draft). Version draft. Washington, D.C. : American Chemical Society. https://doi.org/https://doi.org/10.26434/chemrxiv-2022-svh1x
de Miguel-Jiménez A, Ebeling B, Paez J I, Fink-Straube C, Pearson S, del Campo A. Gelation kinetics of thiol-methylsulfone (MS) hydrogel formulations for 3D cell culture. Published online 2022. doi:https://doi.org/10.26434/chemrxiv-2022-svh1x


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