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Title: Toward light‐regulated living biomaterials
Authors: Sankaran, ShrikrishnanZhao, ShifangMuth, ChristinaPaez, JulietaDel Campo, Aránzazu
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Issue Date: 2018
Published in: Advanced science, Volume 5 (2018), Issue 8
Journal: Advanced Science
Volume: 5
Issue: 8
Publisher: Hoboken, NJ : Wiley
Abstract: Living materials are an emergent material class, infused with the productive,adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)-based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin-specific miniprotein on the outer membrane of an endotoxin-free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light-activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner.
Keywords: dynamic biomaterials endotoxin-free E. coli living biointerfaces optogenetics
Type: article; Text
Publishing status: publishedVersion
DDC: 620
License: CC BY 3.0 Unported
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Appears in Collections:Ingenieurwissenschaften

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Sankaran, Shrikrishnan, Shifang Zhao, Christina Muth, Julieta Paez and Aránzazu Del Campo, 2018. Toward light‐regulated living biomaterials. 2018. Hoboken, NJ : Wiley
Sankaran, S., Zhao, S., Muth, C., Paez, J. and Del Campo, A. (2018) “Toward light‐regulated living biomaterials.” Hoboken, NJ : Wiley. doi:
Sankaran S, Zhao S, Muth C, Paez J, Del Campo A. Toward light‐regulated living biomaterials. Vol. 5. Hoboken, NJ : Wiley; 2018.
Sankaran, S., Zhao, S., Muth, C., Paez, J., & Del Campo, A. (2018). Toward light‐regulated living biomaterials (Version publishedVersion, Vol. 5). Version publishedVersion, Vol. 5. Hoboken, NJ : Wiley.
Sankaran S, Zhao S, Muth C, Paez J, Del Campo A. Toward light‐regulated living biomaterials. 2018;5(8). doi:

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