Design of biomimetic collagen matrices by reagent-free electron beam induced crosslinking: Structure-property relationships and cellular response

dc.bibliographicCitation.firstPage107606
dc.bibliographicCitation.journalTitleMaterials and designeng
dc.bibliographicCitation.volume168
dc.contributor.authorRiedel, Stefanie
dc.contributor.authorHietschold, Philine
dc.contributor.authorKrömmelbein, Catharina
dc.contributor.authorKunschmann, Tom
dc.contributor.authorKonieczny, Robert
dc.contributor.authorKnolle, Wolfgang
dc.contributor.authorMierke, Claudia T.
dc.contributor.authorZink, Mareike
dc.contributor.authorMayr, Stefan G.
dc.date.accessioned2022-08-22T06:03:05Z
dc.date.available2022-08-22T06:03:05Z
dc.date.issued2019
dc.description.abstractNovel strategies to mimic mammalian extracellular matrix (ECM) in vitro are desirable to study cell behavior, diseases and new agents in drug delivery. Even though collagen represents the major constituent of mammalian ECM, artificial collagen hydrogels with characteristic tissue properties such as network size and stiffness are difficult to design without application of chemicals which might be even cytotoxic. In our study we investigate how high energy electron induced crosslinking can be utilized to precisely tune collagen properties for ECM model systems. Constituting a minimally invasive approach, collagen residues remain intact in the course of high energy electron treatment. Quantification of the 3D pore size of the collagen network as a function of irradiation dose shows an increase in density leading to decreased pore size. Rheological measurements indicate elevated storage and loss moduli correlating with an increase in crosslinking density. In addition, cell tests show well maintained viability of NIH 3T3 cells for irradiated collagen gels indicating excellent cellular acceptance. With this, our investigations demonstrate that electron beam crosslinked collagen matrices have a high potential as precisely tunable ECM-mimetic systems with excellent cytocompatibility.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/10098
dc.identifier.urihttp://dx.doi.org/10.34657/9136
dc.language.isoengeng
dc.publisherAmsterdam [u.a.] : Elsevier Science
dc.relation.doihttps://doi.org/10.1016/j.matdes.2019.107606
dc.relation.essn0264-1275
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc600
dc.subject.ddc690
dc.subject.otherBiomimetic collageneng
dc.subject.otherExtracellular matrix modeleng
dc.subject.otherHigh energy electron crosslinkingeng
dc.subject.otherReagent-free crosslinkingeng
dc.titleDesign of biomimetic collagen matrices by reagent-free electron beam induced crosslinking: Structure-property relationships and cellular responseeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIOM
wgl.subjectIngenieurwissenschaftenger
wgl.subjectPhysikger
wgl.typeZeitschriftenartikelger
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Design_of_biomimetic.pdf
Size:
2.33 MB
Format:
Adobe Portable Document Format
Description: