Successful optimization of reconstruction parameters in structured illumination microscopy
dc.bibliographicCitation.firstPage | 69 | eng |
dc.bibliographicCitation.journalTitle | Optics communications | eng |
dc.bibliographicCitation.lastPage | 75 | eng |
dc.bibliographicCitation.volume | 436 | eng |
dc.contributor.author | Karras, Christian | |
dc.contributor.author | Smedh, Maria | |
dc.contributor.author | Förster, Ronny | |
dc.contributor.author | Deschout, Hendrik | |
dc.contributor.author | Fernandez-Rodriguez, Julia | |
dc.contributor.author | Heintzmann, Rainer | |
dc.date.accessioned | 2021-11-30T07:19:12Z | |
dc.date.available | 2021-11-30T07:19:12Z | |
dc.date.issued | 2019 | |
dc.description.abstract | The impact of the different reconstruction parameters in super-resolution structured illumination microscopy (SIM) on image artifacts is carefully analyzed. These parameters comprise the Wiener filter parameter, an apodization function, zero-frequency suppression and modifications of the optical transfer function. A detailed investigation of the reconstructed image spectrum is concluded to be suitable for identifying artifacts. For this purpose, two samples, an artificial test slide and a more realistic biological system, were used to characterize the artifact classes and their correlation with the image spectra as well as the reconstruction parameters. In addition, a guideline for efficient parameter optimization is suggested and the implementation of the parameters in selected up-to-date processing packages (proprietary and open-source) is depicted. © 2018 The Authors | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/7560 | |
dc.identifier.uri | https://doi.org/10.34657/6607 | |
dc.language.iso | eng | eng |
dc.publisher | Amsterdam [u.a.] : Elsevier | eng |
dc.relation.doi | https://doi.org/10.1016/j.optcom.2018.12.005 | |
dc.relation.essn | 1873-0310 | |
dc.rights.license | CC BY-NC-ND 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | eng |
dc.subject.ddc | 530 | eng |
dc.subject.other | Fluorescence microscopy | eng |
dc.subject.other | Image reconstruction | eng |
dc.subject.other | Parameter estimation | eng |
dc.subject.other | Structured illumination microscopy | eng |
dc.subject.other | Super resolution | eng |
dc.title | Successful optimization of reconstruction parameters in structured illumination microscopy | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPHT | eng |
wgl.subject | Physik | eng |
wgl.type | Zeitschriftenartikel | eng |
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