An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020
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Date
2020
Volume
25
Issue
25
Journal
Series Titel
Book Title
Publisher
Stockholm : European Centre for Disease Prevention and Control
Link to publishers version
Abstract
We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.
Description
Keywords
BD MAX, next generation sequencing, CC1-MRSA-IV, Cepheid GenXpert, commercial PCR, Staphylococci, Staphylococcus aureus
Citation
Monecke, S., König, E., Earls, M. R., Leitner, E., Müller, E., Wagner, G. E., et al. (2020). An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020. 25(25). https://doi.org//10.2807/1560-7917.ES.2020.25.25.2000929
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License
CC BY 4.0 Unported